Fig. 4

Different regulation of the glomerular structure through Elmo1, Elmo2 and Elmo3 in the kidney of adult zebrafish. (A) Representative light microscopy images of PAS stained sections of glomeruli in the kidney of elmo1 ^+/+ and elmo1 ^−/− , elmo2 ^+/+ and elmo2 ^−/− and elmo3 ^+/+ and elmo3 ^−/− adult zebrafish (9–13 mpf). Scale bar is 20 µm. (B) Analysis of the glomerulus structure in adult zebrafish kidneys showed no changes in the number of nuclei, glomerulus size and capillary area but an increase in the bowman space in elmo1 ^−/− compared to elmo1 ^+/+ . n = 35 glomeruli per group, always seven glomeruli per kidney. (C) Analysis of the glomerulus structure in adult zebrafish kidneys showed no changes in the number of nuclei and the capillary area but an increase in glomerulus size and bowman space in elmo2 ^−/− compared to elmo2 ^+/+ . n = 20 glomeruli per group, always five per kidney. (D) Analysis of the glomerulus structure in adult zebrafish kidneys showed no alterations in elmo3 ^−/− compared to elmo3 ^+/+ . n = 32 glomeruli per group, always eight per kidney. Statistical analysis was done with t-test and Mann-Whitney test. *p < 0.05, **p < 0.01. ns, not significant; mpf, months post fertilization.