Fig. 3

SCFAs restore endocytic function but not mucus production. A. Representative images of the mid-intestine of larvae at 10 dpf exposed to Neutral Red vital staining. Rostral to the left and dorsal up. Scale bar 100 μm. B. Graph representing the area of neutral red staining in the mid-intestine of 10 dpf larvae. One-way ANOVA with multiple comparisons. ****p < 0.0001. N = 19–32. C. Representative images of the mid-intestine of larvae at 10 dpf stained with Alcian Blue. Rostral to the left and dorsal up. Scale bar 100 μm. D. Graph representing the number of goblet cells stained by Alcian Blue at the mid-intestine of 10 dpf larvae. One-way ANOVA with Tukey's multiple comparisons. **p < 0.01; ****p < 0.0001. N = 42–45. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)