PUBLICATION

Short chain fatty acids (SCFAs) improves TNBS-induced colitis in zebrafish

Authors
Morales Fénero, C., Amaral, M.A., Xavier, I.K., Padovani, B.N., Paredes, L.C., Takiishi, T., Lopes-Ferreira, M., Lima, C., Colombo, A., Saraiva Câmara, N.O.
ID
ZDB-PUB-220503-8
Date
2021
Source
Current research in immunology   2: 142-154 (Journal)
Registered Authors
Keywords
Intestinal inflammation, Larval zebrafish, Microbiota, Short-chain fatty acids, TNBS
MeSH Terms
none
PubMed
35492385 Full text @ Curr Res Immunol
Abstract
The short-chain fatty acids (SCFAs) are metabolites originated from the fermentation of dietary fibers and amino acids produced by the bacteria of the intestinal microbiota. The most abundant SCFAs, acetate, propionate, and butyrate, have been proposed as a treatment for inflammatory bowel diseases (IBDs) due to their anti-inflammatory properties. This work aimed to analyze the effects of the treatment of three combined SCFAs in TNBS-induced intestinal inflammation in zebrafish larvae. Here, we demonstrated that SCFAs significantly increased the survival of TNBS-exposed larvae, preserved the intestinal endocytic function, reduced the expression of inflammatory cytokines and the intestinal recruitment of neutrophils caused by TNBS. However, SCFAs treatment did not appear to avoid TNBS-induced tissue damage in the intestinal wall and did not restore the number of mucus-producing goblet cells. Finally, exposure to TNBS induced dysbiosis of the microbiota with an increase in Betaproteobacteria and Actinobacteria, while the treatment with SCFAs maintained these population levels similar to control. Thus, we demonstrate that the treatment of three combined SCFAs presented anti-inflammatory properties previously seen in mammals, opening an opportunity to use zebrafish to explore the potential benefit of these and other metabolites to treat inflammation.
Genes / Markers
Figures
Figure Gallery (5 images)
Show all Figures
Expression
Phenotype
Fish Conditions Stage Phenotype Figure
ABchemical treatment by environment: 2,4,6-trinitrobenzenesulfonic acidDays 7-13
ABchemical treatment by environment: 2,4,6-trinitrobenzenesulfonic acidDays 7-13
ABchemical treatment by environment: 2,4,6-trinitrobenzenesulfonic acidDays 7-13
ABchemical treatment by environment: 2,4,6-trinitrobenzenesulfonic acidDays 7-13
ABchemical treatment by environment: 2,4,6-trinitrobenzenesulfonic acidDays 7-13
ABchemical treatment by environment: 2,4,6-trinitrobenzenesulfonic acidDays 7-13
ABchemical treatment by environment: 2,4,6-trinitrobenzenesulfonic acidDays 7-13
ABchemical treatment by environment: short-chain fatty acid, chemical treatment by environment: 2,4,6-trinitrobenzenesulfonic acidDays 7-13
ABchemical treatment by environment: short-chain fatty acid, chemical treatment by environment: 2,4,6-trinitrobenzenesulfonic acidDays 7-13
ABchemical treatment by environment: short-chain fatty acid, chemical treatment by environment: 2,4,6-trinitrobenzenesulfonic acidDays 7-13
1 - 10 of 19
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
nz50TgTransgenic Insertion
    1 - 1 of 1
    Show
    Human Disease / Model
    Human Disease Fish Conditions Evidence
    colitisABchemical treatment by environment: 2,4,6-trinitrobenzenesulfonic acidTAS
    1 - 1 of 1
    Show
    Sequence Targeting Reagents
    No data available
    Fish
    Fish
    AB
    nz50Tg
    1 - 2 of 2
    Show
    Antibodies
    No data available
    Orthology
    No data available
    Engineered Foreign Genes
    Marker Marker Type Name
    DsRed2EFGDsRed2
    1 - 1 of 1
    Show
    Mapping
    No data available
    Your Input Welcome
    We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
    Citation
    Morales Fénero, C., Amaral, M.A., Xavier, I.K., Padovani, B.N., Paredes, L.C., Takiishi, T., Lopes-Ferreira, M., Lima, C., Colombo, A., Saraiva Câmara, N.O. (2021) Short chain fatty acids (SCFAs) improves TNBS-induced colitis in zebrafish. Current research in immunology. 2:142-154.