FIGURE

Fig. 5

ID

ZDB-FIG-220628-153

Publication

He et al., 2021 - Zebrafish foxc1a controls ventricular chamber maturation by directly regulating wwtr1 and nkx2.5 expression

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Fig. 5

Fig. 5. The defects observed in the foxc1a nju18 ventricle can be partially abated by the conditional knockin of nkx2.5 in foxc1a+ cells. A: The WISH results reveal that the expression of nkx2.5 is rescued in foxc1a nju81 hearts. Sample size: WT (n = 10), foxc1a nju81 (n = 15) at 6 hpf; WT (n = 9), foxc1a nju81 (n = 6) at 72 hpf; WT (n = 14), foxc1a nju81 (n = 7) at 96 hpf. The 6 hpf embryos are lateral views with the animal pole oriented upward. The 72 and 96 hpf embryos are ventral views with the head oriented upward. B: Maximum intensity projections of confocal z-stacks show representative hearts of WT (n = 19) and foxc1a nju81 (n = 15) embryos at 96 hpf. C: A scatter plot shows the numbers of ventricular cells. D: Lateral views of WT, foxc1a nju18, and foxc1a nju81 embryos. a, The distance from the center of the ventricle to the edge of the heart; b, the distance from the center of the ventricle to the edge of the pericardium. E: A scatter plot shows the pericardial edema indexes of the WT (n = 20), foxc1a nju18 (n = 29), and foxc1a nju81 (n = 33) embryos at 108 hpf. F: Representative hearts of foxc1a nju18 and foxc1a nju81 embryos at 96 hpf. G: A scatter plot shows the SF in WT (n = 21), foxc1a nju18 (n = 25), and foxc1a nju81 (n = 21) embryos. ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001; ∗∗∗∗, P < 0.0001; ns, P > 0.05; Error bars represent the standard deviation. Scale bars, 50 μm (A, B, F); 100 μm (D). V, ventricle; A, atrium; VD, ventricular diastole; VS, ventricular systole.

Expression Data

Genes:	DsRed GFP nkx2.5
Fish:	TU f1Tg zf3348Tg foxc1a^nju18/nju18; f1Tg foxc1a^{nju81Tg/nju81Tg} foxc1a^{nju81Tg/nju81Tg}; f1Tg foxc1a^{nju81Tg/nju81Tg}; zf3348Tg
Anatomical Terms:	atrial myocardium heart presumptive paraxial mesoderm ventricular myocardium ventricular myocardium nucleus
Stage Range:	Shield to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	foxc1a^nju18/nju18 foxc1a^nju18/nju18; f1Tg foxc1a^{nju81Tg/nju81Tg} foxc1a^{nju81Tg/nju81Tg}; f1Tg foxc1a^{nju81Tg/nju81Tg}; zf3348Tg
Observed In:	cardiac muscle cell cardiac ventricle heart heart contraction presumptive paraxial mesoderm
Stage Range:	Shield to Day 4

Phenotype Detail

Acknowledgments

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Reprinted from Journal of genetics and genomics = Yi chuan xue bao, 49(6), He, L., Zhang, Q., Jiang, D., Zhang, Y., Wei, Y., Yang, Y., Li, N., Wang, S., Yue, Y., Zhao, Q., Zebrafish foxc1a controls ventricular chamber maturation by directly regulating wwtr1 and nkx2.5 expression, 559-568, Copyright (2021) with permission from Elsevier. Full text @ J. Genet. Genomics