Fig. 5 Fig. 5. The defects observed in the foxc1a nju18 ventricle can be partially abated by the conditional knockin of nkx2.5 in foxc1a+ cells. A: The WISH results reveal that the expression of nkx2.5 is rescued in foxc1a nju81 hearts. Sample size: WT (n = 10), foxc1a nju81 (n = 15) at 6 hpf; WT (n = 9), foxc1a nju81 (n = 6) at 72 hpf; WT (n = 14), foxc1a nju81 (n = 7) at 96 hpf. The 6 hpf embryos are lateral views with the animal pole oriented upward. The 72 and 96 hpf embryos are ventral views with the head oriented upward. B: Maximum intensity projections of confocal z-stacks show representative hearts of WT (n = 19) and foxc1a nju81 (n = 15) embryos at 96 hpf. C: A scatter plot shows the numbers of ventricular cells. D: Lateral views of WT, foxc1a nju18, and foxc1a nju81 embryos. a, The distance from the center of the ventricle to the edge of the heart; b, the distance from the center of the ventricle to the edge of the pericardium. E: A scatter plot shows the pericardial edema indexes of the WT (n = 20), foxc1a nju18 (n = 29), and foxc1a nju81 (n = 33) embryos at 108 hpf. F: Representative hearts of foxc1a nju18 and foxc1a nju81 embryos at 96 hpf. G: A scatter plot shows the SF in WT (n = 21), foxc1a nju18 (n = 25), and foxc1a nju81 (n = 21) embryos. ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001; ∗∗∗∗, P < 0.0001; ns, P > 0.05; Error bars represent the standard deviation. Scale bars, 50 μm (A, B, F); 100 μm (D). V, ventricle; A, atrium; VD, ventricular diastole; VS, ventricular systole.
Reprinted from Journal of genetics and genomics = Yi chuan xue bao, 49(6), He, L., Zhang, Q., Jiang, D., Zhang, Y., Wei, Y., Yang, Y., Li, N., Wang, S., Yue, Y., Zhao, Q., Zebrafish foxc1a controls ventricular chamber maturation by directly regulating wwtr1 and nkx2.5 expression, 559-568, Copyright (2021) with permission from Elsevier. Full text @ J. Genet. Genomics