ZFIN Figure: Yang et al., 2022, Fig. 1

ZFIN ID: ZDB-FIG-220622-95

ZFIN ID: ZDB-FIG-220622-95

Yang et al., 2022 - Myoneurin regulates BMP signaling by competing with Ppm1a for Smad binding. iScience 25:104495 Full text @ iScience

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Antibodies:	Ab1-col2a Ab4-GFP
Fish:	mynn^{ioz054Gt/ioz054Gt}
Anatomical Term:	pharyngeal arch
Stage Range:	Long-pec to Protruding-mouth

PHENOTYPE:

Fish:	mynn^{ioz054Gt/ioz054Gt}
Observed In:	ceratobranchial cartilage ceratohyal cartilage eye head hyosymplectic cartilage integument Meckel's cartilage palatoquadrate arch palatoquadrate cartilage pericardium post-vent region ventral mandibular arch
Stage Range:	Long-pec to Day 4

Fig. 1

The GFP expression and developmental defects in T054 embryos

(A) Morphology of T054 mutants at indicated stages. Note that mutant embryos had a darker head, rough skin, reduced size of head and eyes, severe craniofacial jaw malformations, and curly posterior trunk with stage-dependent degrees. Scale bars, 200 μm.

(B) Fluorescent images of T054 embryos. The pictured embryos were heterozygotes. Scale bars, 200 μm.

(C) Immunostaining of chondrocytes by anti-Col2 and GFP antibody. T054 heterozygous embryos at 72 hpf were co-immunostained with anti-Col2 (red) and anti-GFP (green) antibodies. Scale bar, 20 μm.

(D) Craniofacial cartilages stained with Alcian blue. Note that almost no cartilages could be seen in the T054 mutant embryos. The cartilages were positioned with anterior to the left, and ceratobranchial (cb), ceratohyal (ch), Meckel’s cartilage (m), palatoquadrate (pq), and hyosymplectic (hs) cartilages were shown. Scale bars, 200 μm.

Gene Expression Details No data available

Antibody Labeling Details

Antibody	Assay	Fish	Conditions	Stage	Anatomy
Ab1-col2a	IHC	mynn^{ioz054Gt/ioz054Gt}	standard conditions	Protruding-mouth	pharyngeal arch
Ab4-GFP	IHC	mynn^{ioz054Gt/ioz054Gt}	standard conditions	Long-pec	pharyngeal arch
	IHC			Protruding-mouth	pharyngeal arch

Phenotype Details

Fish	Conditions	Stage	Phenotype
mynn^{ioz054Gt/ioz054Gt}	standard conditions	Long-pec	eye decreased size, abnormal
		Long-pec	head decreased size, abnormal
		Long-pec	integument rough, abnormal
		Protruding-mouth	eye decreased size, abnormal
		Protruding-mouth	head decreased size, abnormal
		Protruding-mouth	integument rough, abnormal
		Protruding-mouth	pericardium edematous, abnormal
		Protruding-mouth	post-vent region curved, abnormal
		Day 4	ceratobranchial cartilage absence of anatomical entity, abnormal
		Day 4	ceratohyal cartilage absence of anatomical entity, abnormal
		Day 4	eye decreased size, abnormal
		Day 4	head decreased size, abnormal
		Day 4	hyosymplectic cartilage absence of anatomical entity, abnormal
		Day 4	integument rough, abnormal
		Day 4	Meckel's cartilage absence of anatomical entity, abnormal
		Day 4	palatoquadrate arch absence of anatomical entity, abnormal
		Day 4	palatoquadrate cartilage absence of anatomical entity, abnormal
		Day 4	pericardium edematous, abnormal
		Day 4	post-vent region curved, abnormal
		Day 4	ventral mandibular arch absence of anatomical entity, abnormal

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ iScience