ZFIN ID: ZDB-FIG-220622-95
Yang et al., 2022 - Myoneurin regulates BMP signaling by competing with Ppm1a for Smad binding. iScience   25:104495 Full text @ iScience
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Term:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Day 4

Fig. 1

The GFP expression and developmental defects in T054 embryos

(A) Morphology of T054 mutants at indicated stages. Note that mutant embryos had a darker head, rough skin, reduced size of head and eyes, severe craniofacial jaw malformations, and curly posterior trunk with stage-dependent degrees. Scale bars, 200 μm.

(B) Fluorescent images of T054 embryos. The pictured embryos were heterozygotes. Scale bars, 200 μm.

(C) Immunostaining of chondrocytes by anti-Col2 and GFP antibody. T054 heterozygous embryos at 72 hpf were co-immunostained with anti-Col2 (red) and anti-GFP (green) antibodies. Scale bar, 20 μm.

(D) Craniofacial cartilages stained with Alcian blue. Note that almost no cartilages could be seen in the T054 mutant embryos. The cartilages were positioned with anterior to the left, and ceratobranchial (cb), ceratohyal (ch), Meckel’s cartilage (m), palatoquadrate (pq), and hyosymplectic (hs) cartilages were shown. Scale bars, 200 μm.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-col2a IHC mynnioz054Gt/ioz054Gt standard conditions Protruding-mouth pharyngeal arch
Ab4-GFP IHC mynnioz054Gt/ioz054Gt standard conditions Long-pec pharyngeal arch
IHC Protruding-mouth pharyngeal arch
Phenotype Details
Fish Conditions Stage Phenotype
mynnioz054Gt/ioz054Gt standard conditions Long-pec eye decreased size, abnormal
Long-pec head decreased size, abnormal
Long-pec integument rough, abnormal
Protruding-mouth eye decreased size, abnormal
Protruding-mouth head decreased size, abnormal
Protruding-mouth integument rough, abnormal
Protruding-mouth pericardium edematous, abnormal
Protruding-mouth post-vent region curved, abnormal
Day 4 ceratobranchial cartilage absence of anatomical entity, abnormal
Day 4 ceratohyal cartilage absence of anatomical entity, abnormal
Day 4 eye decreased size, abnormal
Day 4 head decreased size, abnormal
Day 4 hyosymplectic cartilage absence of anatomical entity, abnormal
Day 4 integument rough, abnormal
Day 4 Meckel's cartilage absence of anatomical entity, abnormal
Day 4 palatoquadrate arch absence of anatomical entity, abnormal
Day 4 palatoquadrate cartilage absence of anatomical entity, abnormal
Day 4 pericardium edematous, abnormal
Day 4 post-vent region curved, abnormal
Day 4 ventral mandibular arch absence of anatomical entity, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ iScience