FIGURE

Fig. 7

ID

ZDB-FIG-220622-119

Publication

Djokic et al., 2022 - Novel Quorum Quenching YtnP Lactonase From Bacillus paralicheniformis Reduces Pseudomonas aeruginosa Virulence and Increases Antibiotic Efficacy in vivo

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Fig. 7

In vivo toxicity evaluation of YtnP-ZP1, tobramycin, and their combination in the zebrafish (Danio rerio) model. Acute toxicity was assessed using wild-type embryos exposed to the different doses of enzyme, antibiotic, and their combination (A). Embryos were treated at 6 hpf and evaluated for survival, teratogenicity, cardiotoxicity, and hepatotoxicity at 120 hpf (n = 60 per a dose). Normally developed embryos, without signs of hepato- and cardiotoxicity, after exposure to 100 μg mL^–1 of YtnP-ZP1, 2 × MIC of tobramycin and the combination of 100 μg mL^–1 lactonase and 2 × MIC tobramycin are shown (B). The normal (clear) liver in a control embryo is outlined. Tetracycline caused liver necrosis and darkening (dashed outlined), yolk necrosis (asterisk), and pericardial edema (arrow). The hepatotoxicity of the tested enzyme and its combination with antibiotic was additionally verified in zebrafish embryos with fluorescently labeled liver (n = 6) according to the liver fluorescence (C) and the liver area index (D).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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