Figure 3. The Dvl and ETTV motifs act in a partially redundant manner during zebrafish brain angiogenesis (A) Scheme of zebrafish Gpr124 highlighting the CRISPR-Cas9 target site corresponding to the gpr124ulb14(?ETTV) mutant allele. (B) Lateral views of WT and ?ETTV?/? mutant embryos at 60 hpf. (C) Lateral views of the skin-pigmentation patterns of WT and ?ETTV?/? mutant adults. (D) Dorsal views of hindbrain vasculature of WT and ?ETTV?/? mutants. (E) Quantification of CtAs of WT and ?ETTV?/? mutant embryos at 48 hpf. (F) Same as (E) at 60 hpf. (G) 3D representation of cerebrovasculature of 60 hpf WT and ?ETTV mutant embryos. (H) Same as (E) in 5 dpf larvae. (I) Quantification of the number of lumenized CtAs in 48 hpf WT and ?ETTV mutant embryos. (J and K) Dorsal views (J) and quantification (K) of dorsal root ganglia in the trunk region of WT and ?ETTV mutant larvae at 72 hpf. (L) Luciferase activities of HEK293 STF cells co-transfected with Wnt7a, Fz1, Reck, and increasing amounts of the indicated Gpr124 ICD variants; n = 3. (M) Quantification of hindbrain CtAs of gpr124 morphant embryos at 48 hpf injected at the one-cell stage with 100 pg of the indicated mRNAs. Scale bars, 0.5 mm for (B) and 100 ?m for (D), (G), and (J). Related to Figure S1.
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