ZFIN ID: ZDB-FIG-220521-7
Espinosa et al., 2022 - Characterization of a novel zebrafish model of SPEG-related centronuclear myopathy. Disease models & mechanisms   15(5) Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Day 5 to Days 7-13
PHENOTYPE:
Fish:
Observed In:
Stage Range: Day 5 to Days 7-13

Fig. 7.

Dnm2 protein is upregulated in both speg-DKO and mtm1-KO zebrafish. (A-C) Myofibers were isolated at 5 dpf (speg-DKO) and 7 dpf (mtm1-KO), and stained with anti-Dnm2 (GTX127330, GeneTex). (A) In WT myofibers (5 dpf), Dnm2 is localized to the triads (transverse striations). (B,C) Similar striated patterns can be observed for Dnm2 in speg-DKO (B) and mtm1-KO (C), with occasional Dnm2 aggregations observed along the striations (yellow arrowheads). Scale bars: 10 µm. (D-E′) Western blot analysis shows increased Dnm2 protein levels in 5 dpf speg-DKO (D,D′) and 7 dpf mtm1-KO (E,E′) compared to WT. Each lane (D,E) or each dot (D′,E′) represents n=25 zebrafish (40 µg total protein). Densitometry was accomplished using Fiji ImageJ. Dnm2 protein level was normalized to β-actin loading controls in speg-DKO, or to REVERT total protein stains in mtm1-KO (as β-actin level is changed by the lack of Mtm1). Student's t-test: *P<0.05; **P<0.01; ns, not significant.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-dnm2 IHC AB standard conditions Day 5 skeletal muscle cell junctional membrane complex
IHC mtm1zf711/zf711 (AB) standard conditions Days 7-13 skeletal muscle cell cytoplasm
IHC Days 7-13 skeletal muscle cell junctional membrane complex
IHC spegahsc191/hsc191; spegbhsc192/hsc192 (AB) standard conditions Day 5 skeletal muscle cell cytoplasm
IHC Day 5 skeletal muscle cell junctional membrane complex
WB AB standard conditions Day 5 whole organism
WB Days 7-13 whole organism
WB mtm1zf711/zf711 (AB) standard conditions Days 7-13 whole organism
WB spegahsc191/hsc191; spegbhsc192/hsc192 (AB) standard conditions Day 5 whole organism
Phenotype Details
Fish Conditions Stage Phenotype
mtm1zf711/zf711 (AB) standard conditions Days 7-13 skeletal muscle cell cytoplasm Ab1-dnm2 labeling increased amount, abnormal
Days 7-13 skeletal muscle cell cytoplasm Ab1-dnm2 labeling spatial pattern, abnormal
Days 7-13 whole organism Ab1-dnm2 labeling increased amount, abnormal
spegahsc191/hsc191; spegbhsc192/hsc192 (AB) standard conditions Day 5 skeletal muscle cell cytoplasm Ab1-dnm2 labeling increased amount, abnormal
Day 5 skeletal muscle cell cytoplasm Ab1-dnm2 labeling spatial pattern, abnormal
Day 5 whole organism Ab1-dnm2 labeling increased amount, abnormal
Acknowledgments:
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