FIGURE 3

Kinase inhibitors active in transplanted ZMEL1 melanoma cells. (A) Inhibitors that targeted the RAS and p38 MAPK pathways and significantly inhibited melanoma cell growth in vivo. (B) Inhibitors that targeted cell cycle related proteins and significantly inhibited melanoma cell growth in vivo. (C) Inhibitors that targeted receptor tyrosine kinases (RTKs) and significantly inhibited melanoma cell growth in vivo. (A–C) Statistical significance was determined by Mann-Whitney test. *p < 0.04, **p < 0.002, ***p < 0.001. Luminescence measured in vivo in single embryos is represented by a single dot in dot plots. All experiments were done in 2–3 repeats. (D) List of all the inhibitors from A-C with their predicted main protein targets in zebrafish ZMEL1 cells. The last column with red bars on the right represents the average expression of individual target genes in zebrafish cells which was extracted from a publicly available RNA-sequencing dataset. Inhibitors with a potent new function in melanoma are labeled with a red asterisk.