FIGURE

Fig. 1

ID

ZDB-FIG-220513-30

Publication

Hortle et al., 2022 - OXSR1 inhibits inflammasome activation by limiting potassium efflux during mycobacterial infection

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Fig. 1

Figure 1. Infection-induced OXSR1 aids the growth of M. marinum in zebrafish.

(A) Relative expression of oxsr1a and stk39 in zebrafish embryos at 3 dpi with WT M. marinum, compared with age-matched uninfected controls. Biological replicates (n = 6) each represent pooled RNA from 7 to 10 embryos. (B) Representative images of M. marinum–tdTomato (red) bacterial burden in WT and mosaic F0 oxsr1a crispant embryos. (C) Quantification of M. marinum bacterial burden in WT and mosaic F0 oxsr1a crispant embryos and F0 stk39 crispant embryos. Each graph shows combined results of two independent experiments. (D) Quantification of M. marinum bacterial burden in WT, heterozygous and homozygous oxsr1a knockout embryos. Graph shows combined results of two independent experiments. (E) Quantification of WT M. marinum bacterial burden in DMSO vehicle control and Compound B-treated embryos at 1.8 μM. (F) Quantification of axenic M. marinum–tdTomato growth by fluorescence in 7H9 broth culture supplemented with Compound B. Red line (1.8 μM) indicates the concentration used to treat infected embryos.

Expression Data

Genes:	oxsr1a stk39
Fish:	WT
Condition:	bacterial treatment by exposure to environment: Mycobacterium marinum
Anatomical Term:	whole organism
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT oxsr1a^syd5/syd5 WT + CRISPR1-oxsr1a + CRISPR2-oxsr1a + CRISPR3-oxsr1a + CRISPR4-oxsr1a WT + CRISPR1-stk39 + CRISPR2-stk39 + CRISPR3-stk39 + CRISPR4-stk39 oxsr1a^syd5/+
Conditions:	bacterial treatment by exposure to environment: Mycobacterium marinum, chemical treatment by environment: 7-ethoxy-N(3)-(2-furanylmethyl)-3,9-acridinediamine bacterial treatment by exposure to environment: Mycobacterium marinum
Knockdown Reagents:	CRISPR1-oxsr1a CRISPR1-stk39 CRISPR2-oxsr1a CRISPR2-stk39 CRISPR3-oxsr1a CRISPR3-stk39 CRISPR4-oxsr1a CRISPR4-stk39
Observed In:	defense response to bacterium whole organism
Stage Range:	Protruding-mouth to Day 5

Phenotype Detail

Acknowledgments

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