ZFIN Figure: Shibata et al., 2022, Figure 6

Figure 6

Lipidomic analyses of lpgat1 mutant zebrafish. Lipids were collected at 7 days post fertilization from a single zebrafish larva obtained from crossing an lpgat1^+/− male with an lpgat1^+/− female and analyzed for phosphatidylcholine (PC, A), phosphatidylethanolamine (PE, B), phosphatidylinositol (PI, C), phosphatidylserine (PS, D), phosphatidylglycerol (PG, E), and phosphatidic acid (PA, F) by LC–MS/MS. Genotypes of each larva were determined by PCR after a lipid sample was collected. The fish were obtained from three independent crosses. The numbers of samples were n = 18 for lpgat1^+/+, n = 19 for lpgat1^+/− and n = 7 for lpgat1^−/−. Error bars are S.D. Statistically significant differences are marked with asterisks. *p < 0.05; **p < 0.01; ***p < 0.001. Two-way ANOVA, Bonferroni’s multiple comparison test was used. For molecular species with low abundance, the relative abundance of each molecular species is shown in Supplementary Fig. S9.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	lpgat1^{zf3665/zf3665} (AB) lpgat1^{zf3666/zf3666} (AB) lpgat1^zf3665/+ (AB) lpgat1^zf3666/+ (AB)
Observed In:	whole organism phosphatidic acid 36:2 whole organism phosphatidic acid 36:3 whole organism phosphatidic acid 36:4 whole organism phosphatidic acid 38:4 whole organism phosphatidic acid 40:6 whole organism phosphatidylcholine 32:0 whole organism phosphatidylcholine 32:1 whole organism phosphatidylcholine 34:0 whole organism phosphatidylcholine 34:1 whole organism phosphatidylcholine 34:2 whole organism phosphatidylcholine 36:1 whole organism phosphatidylcholine 36:2 whole organism phosphatidylcholine 36:3 whole organism phosphatidylcholine 36:4 whole organism phosphatidylcholine 38:4 whole organism phosphatidylcholine 38:5 whole organism phosphatidylcholine 38:6 whole organism phosphatidylcholine 40:5 whole organism phosphatidylcholine 40:6 whole organism phosphatidylcholine 40:7 whole organism phosphatidylethanolamine 32:0 whole organism phosphatidylethanolamine 32:1 zwitterion whole organism phosphatidylethanolamine 34:0 zwitterion whole organism phosphatidylethanolamine 34:1 whole organism phosphatidylethanolamine 34:2 whole organism phosphatidylethanolamine 36:1 whole organism phosphatidylethanolamine 36:2 whole organism phosphatidylethanolamine 36:3 whole organism phosphatidylethanolamine 36:4 whole organism phosphatidylethanolamine 38:3 whole organism phosphatidylethanolamine 38:4 whole organism phosphatidylethanolamine 38:5 whole organism phosphatidylethanolamine 38:6 whole organism phosphatidylethanolamine 40:5 whole organism phosphatidylethanolamine 40:6 whole organism phosphatidylethanolamine 40:7 whole organism phosphatidylglycerol 32:0 whole organism phosphatidylglycerol 32:1 whole organism phosphatidylglycerol 34:0 whole organism phosphatidylglycerol 34:1 whole organism phosphatidylglycerol 34:2 whole organism phosphatidylglycerol 36:1 whole organism phosphatidylglycerol 36:2 whole organism phosphatidylglycerol 36:3 whole organism phosphatidylglycerol 38:3 whole organism phosphatidylglycerol 38:4 whole organism phosphatidylglycerol 38:5 whole organism phosphatidylglycerol 38:6 whole organism phosphatidylglycerol 40:5 whole organism phosphatidylglycerol 40:6 whole organism phosphatidylglycerol 40:7 whole organism phosphatidylinositol 34:0 whole organism phosphatidylinositol 34:1 whole organism phosphatidylinositol 34:2 whole organism phosphatidylinositol 36:1 whole organism phosphatidylinositol 36:2 whole organism phosphatidylinositol 36:3 whole organism phosphatidylinositol 36:4 whole organism phosphatidylinositol 38:3 whole organism phosphatidylinositol 38:4 whole organism phosphatidylinositol 38:5 whole organism phosphatidylinositol 38:6 whole organism phosphatidylinositol 40:5 whole organism phosphatidylinositol 40:6 whole organism phosphatidylinositol 40:7 whole organism phosphatidylserine 32:1 whole organism phosphatidylserine 34:0(1-) whole organism phosphatidylserine 34:1 whole organism phosphatidylserine 34:2 whole organism phosphatidylserine 36:1 whole organism phosphatidylserine 36:2 whole organism phosphatidylserine 36:3 whole organism phosphatidylserine 36:4 whole organism phosphatidylserine 38:3 whole organism phosphatidylserine 38:4 whole organism phosphatidylserine 38:5 whole organism phosphatidylserine 38:6 whole organism phosphatidylserine 40:5 whole organism phosphatidylserine 40:6 whole organism phosphatidylserine 40:7
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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