Figure 6.
- ID
- ZDB-FIG-220430-5
- Publication
- Xue et al., 2022 - Cigarette smoke-induced oxidative stress activates NRF2 to mediate fibronectin disorganization in vascular formation
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Figure 6. NRF2 regulated STAT3 at the transcriptional level. (a) qPCR for the expression levels of STAT3 mRNA in CSE-exposed HUVECs treated with or without NRF2 (2.5/5 µM) inhibitor for 24 h. (b) qPCR for the expression levels of stat3 mRNA in CSE-exposed zebrafish treated with or without NRF2 (25/50 µM) inhibitor for 24 h. (c) STAT3 mRNA expressions were examined with NRF2 overexpression in 293FT cells at 48 h. (d–f) Analysis of the STAT3 protein level by immunoblot with NRF2 overexpression in 293FT cells at 48 h. (g) NRF2-binding motifs from the JASPAR database. (h) Schematic representations of the STAT3 promoter region. Two pairs of primers were designed to flank the potential NRF2-binding sites within the STAT3 promoter. (i) Dual-luciferase assays of transcription with or without NRF2 overexpression. Data presented are the mean ± s.e.m. Unpaired Student's t-test, #p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05. |