Figure 2.
- ID
- ZDB-FIG-220430-16
- Publication
- Xue et al., 2022 - Cigarette smoke-induced oxidative stress activates NRF2 to mediate fibronectin disorganization in vascular formation
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Figure 2. NRF2 was stimulated in CSE-induced vascular damage. (a) FACS for ROS generation in HUVECs exposed to CSE for 6 h. (b,c) NRF2 protein expression levels in HUVECs after 24 h of being exposed to CSE. (d,e) Representative immunofluorescence images and Pearson correlation coefficient analysis show nuclear localization of NRF2 and 4′,6-diamidino-2-phenylindole (DAPI) in HUVECs exposed to CSE for 24 h. (f,g) Representative immunofluorescence images and the statistics of average fluorescence intensity for NRF2 nuclear localization in vascular endothelial cells of flk::GFP zebrafish embryos exposed to CSE for 48 h (the arrows indicate the nuclei of the vascular endothelial cells) (n = 10). Data presented are the mean ± s.e.m. Unpaired Student's t-test, #p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05. DCFH, dichlorofluorescein diacetate; FITC, fluorescein isothiocyanate. |