FIGURE

Fig. 1

ID

ZDB-FIG-220429-17

Publication

Matrone et al., 2021 - Fli1⁺ cells transcriptional analysis reveals an Lmo2-Prdm16 axis in angiogenesis

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Fig. 1

Characterization of low- and high-fli1+ cells. (A) qPCR analysis of fli1 gene expression during zebrafish development (from 1 to 120 hpf). (B) Following enzymatic disaggregation of Tg(fli1:EGFP)y1 zebrafish embryos at 72 hpf, two populations of low- and high-fli1+ cells were identified. Wik zebrafish were used to set the gate for fli1− cells. (C and D) Line graphs showing the percentage of low- and high-fli1+ cells at different developmental stages (C) and relative fli1 gene expression (D). (E) Fluorescent images of low- and high-fli1+ cells 24 h after seeded on fibronectin-coated dishes with endothelial growth medium. (F) Table of cell characterization (percent attached cells and cell number increase). n = 3 experiments; ANOVA test followed by Bonferroni post hoc was used to compare means. ***P ≤ 0.001, **P ≤ 0.01, *P ≤ 0.05.

Expression Data

Gene:	fli1
Fish:	y1Tg/y1Tg WT
Anatomical Terms:	blood vessel endothelial cell whole organism
Stage Range:	4-cell to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA