FIGURE

Fig. 4

ID

ZDB-FIG-220411-11

Publication

Abrial et al., 2022 - Latent TGFβ binding proteins 1 and 3 protect the larval zebrafish outflow tract from aneurysmal dilatation

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Fig. 4

Hyperactivation of canonical TGFβ signaling in the distended OFTs of ltbp1, ltbp3 DKO larvae. (A,B,D,E) Confocal images of OFTs in 5 dpf (A,B) and 3 dpf (D,E) control (CTRL; A,D) and ltbp1^−/−; ltbp3^−/− (B,E) larvae double immunostained for striated muscle (MF20, red) and phosphorylated Smad3 (pSmad3; green). (C,F) Dot plots showing the relative mean pSmad3 fluorescence intensities in the OFTs of 5 dpf (C) or 3 dpf (F) CTRL (n=9 in C; n=6 in F) and ltbp1^−/−; ltbp3^−/− (n=9 in C; n=3 in F) larvae. For C and F, statistical significance was determined with an unpaired t-test. Error bars indicate one standard deviation. ***P<0.01; ns, not significant. Scale bars: 20 µm.

Expression Data

Antibodies:	Ab-MF20 Ab2-smad3
Fish:	WT ltbp1^fb29/fb29; ltbp3^fb28/fb28
Anatomical Terms:	bulbus arteriosus nucleus ventricular myocardium
Stage Range:	Protruding-mouth to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ltbp1^fb29/fb29; ltbp3^fb28/fb28
Observed In:	bulbus arteriosus bulbus arteriosus nucleus bulbus arteriosus transforming growth factor beta receptor signaling pathway cardiac ventricle
Stage Range:	Protruding-mouth to Day 5

Phenotype Detail

Acknowledgments

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