ZFIN ID: ZDB-FIG-220411-11
Abrial et al., 2022 - Latent TGFβ binding proteins 1 and 3 protect the larval zebrafish outflow tract from aneurysmal dilatation. Disease models & mechanisms   15(3) Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. 4

Hyperactivation of canonical TGFβ signaling in the distended OFTs of ltbp1, ltbp3 DKO larvae. (A,B,D,E) Confocal images of OFTs in 5 dpf (A,B) and 3 dpf (D,E) control (CTRL; A,D) and ltbp1−/−; ltbp3−/− (B,E) larvae double immunostained for striated muscle (MF20, red) and phosphorylated Smad3 (pSmad3; green). (C,F) Dot plots showing the relative mean pSmad3 fluorescence intensities in the OFTs of 5 dpf (C) or 3 dpf (F) CTRL (n=9 in C; n=6 in F) and ltbp1−/−; ltbp3−/− (n=9 in C; n=3 in F) larvae. For C and F, statistical significance was determined with an unpaired t-test. Error bars indicate one standard deviation. ***P<0.01; ns, not significant. Scale bars: 20 µm.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-MF20 IHC WT standard conditions Protruding-mouth ventricular myocardium
IHC Day 5 ventricular myocardium
IHC ltbp1fb29/fb29; ltbp3fb28/fb28 standard conditions Protruding-mouth ventricular myocardium
IHC Day 5 ventricular myocardium
Ab2-smad3 IHC WT standard conditions Protruding-mouth bulbus arteriosus nucleus
IHC Day 5 bulbus arteriosus nucleus
IHC ltbp1fb29/fb29; ltbp3fb28/fb28 standard conditions Protruding-mouth bulbus arteriosus nucleus
IHC Day 5 bulbus arteriosus nucleus
Phenotype Details
Fish Conditions Stage Phenotype
ltbp1fb29/fb29; ltbp3fb28/fb28 standard conditions Protruding-mouth bulbus arteriosus normal size, normal
Protruding-mouth bulbus arteriosus transforming growth factor beta receptor signaling pathway normal process quality, normal
Protruding-mouth cardiac ventricle normal size, normal
Day 5 bulbus arteriosus distended, abnormal
Day 5 bulbus arteriosus nucleus ab2-smad3 labeling increased amount, abnormal
Day 5 bulbus arteriosus transforming growth factor beta receptor signaling pathway increased process quality, abnormal
Day 5 cardiac ventricle dilated, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.