Fig. 4

a A SASP factor ROS is detected in mosaic Ras^G12V and Ras^G12VTP53^R175H cells and Ras^G12VTP53^R175H cell neighbours. Representative confocal images show ROS-probe (CellRox DeepRed (magenta))-stained larvae with mosaically introduced cells expressing GFP alone (control) or with Ras^G12V, TP53^R175H, or both Ras^G12V and TP53^R175H (GFP⁺, Ras⁺, TP53^RH+, or Ras⁺TP53^RH+ cells) (green). White arrows indicate ROS in GFP⁺ oncogenic cells. Yellow arrows indicate ROS in the neighbouring area. NAC treatment reduces ROS. Scale bar: 20 μm. Box plots of CellROX intensities in GFP⁺ cells or neighbouring area (20 μm radius around GFP⁺ cells) show first and third quartile, median is represented by a line, whiskers indicate the minimum and maximum, and outliers are shown as dots outside of the box. Each dot represents one cell or one area. A two-tailed one-way ANOVA test with Sidak correction was used. b Ras^G12V and TP53^R175H synergistically promote the expression of SASP factors, interleukin-1β (il1b). Representative confocal images show fluorescence in situ hybridization of il1b mRNA (magenta) in larvae with mosaically introduced cells expressing GFP alone (control) or with Ras^G12V, TP53^R175H, or both Ras^G12V and TP53^R175H (GFP⁺, Ras⁺, TP53^RH+, or Ras⁺TP53^RH+ cells) (green). Scale bar: 20 μm. Bar plots show il1b⁺GFP⁺ cells (mean ± SEM). Each dot represents one larva. A two-tailed one-way ANOVA test with Sidak correction was used. Source data are provided as a Source Data file.