FIGURE

Fig. 2

ID

ZDB-FIG-220328-2

Publication

Kuang et al., 2022 - ALKBH3-dependent m¹A demethylation of Aurora A mRNA inhibits ciliogenesis

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Fig. 2

ALKBH3 removes m<sup>1</sup>A sites on <italic>Aurora A</italic> mRNA.

a Venn diagram showing the overlapped transcripts from the databases of CiliaCarta, m¹A-ID-seq, m¹A-quant-seq, and RNA-seq in ALKBH3-KD cells. KD, knockdown. b Cells infected with lentivirus carrying shRNA targeting negative control (NC) or ALKBH3 were subjected to m¹A RNA immunoprecipitation-qPCR analysis (m¹A-RIP). m¹A levels of Aurora A mRNA in control and ALKBH3-depleted cells were measured. c Peak-calling analysis of specific m¹A peaks of Aurora A mRNA in wild-type and ALKBH3 knockout HEK-293T cells. d Sanger sequencing of Aurora A PCR products amplified from cDNAs that were reverse-transcripted by RT-1306 in the control and ALKBH3-depleted cells. Mutations at the m¹A site are highlighted with red box. e Mutation rates at m¹A sites of Aurora A mRNA in the indicated group are shown. Data are presented as the means ± SD from at least three independent experiments. Student’s t-test; **P < 0.01.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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