Fig. 6

Zebrafish embryos were injected with control morpholino (ctrl MO) or alkbh3 translation-block morpholino (alkbh3 MO) at 1-cell stage and subjected to the following analyses. a, b Bright-field micrographs and statistical analysis of the ciliary phenotypes in alkbh3 morphants. c, d Representative images, and quantification of pronephric ducts in the indicated groups. The lumen of pronephric ducts is indicated by white dashed lines. Scale bar, 10 μm. The widths of pronephric ducts in the indicated groups are measured and the median width of control morphants’ ducts was set at 100%. e Immunostaining of cilia in pronephric ducts in morphants. Cilia were stained by anti-acetylated-α-tubulin antibody (green). The lumen of pronephric ducts is indicated by white dashed lines. Scale bar, 10 μm. f Quantification of embryos with abnormal pronephric cilia. n, the total number of embryos in all experiments. Data are shown as the means ± SD from at least three independent experiments. Student’s t-test; ns, not significant; *P < 0.05, **P < 0.01, ***P < 0.001.