FIGURE

Fig 3

ID

ZDB-FIG-220318-3

Publication

Nandamuri et al., 2022 - Loss of zebrafish dzip1 results in inappropriate recruitment of periocular mesenchyme to the optic fissure and ocular coloboma

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Fig 3

Apoptotic cell death in the optic fissure is not aberrantly upregulated in the <italic toggle='yes'>dzip1</italic> mutant.

(A-F) Whole mount immunofluorescence in wild type (A-C) and dzip1^ts294e mutants (D-F) for cell death (green; activated Caspase-3) and nuclei (magenta; TO-PRO-3) at 24 hpf (A, D), 36 hpf (B, E), and 48 hpf (C, F). All images are lateral views of 3-dimensional renderings. White dashed lines mark the boundary of the eye; yellow dashed lines mark the lens. Examples of cells labeled with activated caspase-3 elsewhere within the eye are indicated (white arrowheads). (G-I) Quantification of total number of activated caspase-3-positive cells within the optic fissure in the eye at 24 hpf, 36 hpf, and 48 hpf. n (embryos) for each genotype shown at the base of the graph. P-values were calculated using Welch’s t-test (M-O). Scale bar: 50 μm. D, dorsal; V, ventral; N, nasal; T, temporal.

Expression Data

Antibody:	Ab1-casp3
Fish:	TU dzip1^{ts294e/ts294e}
Anatomical Terms:	eye immature eye
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dzip1^{ts294e/ts294e}
Observed In:	optic fissure apoptotic process
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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