ZFIN ID: ZDB-FIG-220318-2
Nandamuri et al., 2022 - Loss of zebrafish dzip1 results in inappropriate recruitment of periocular mesenchyme to the optic fissure and ocular coloboma. PLoS One   17:e0265327 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Fig 2 <italic toggle='yes'>dzip1</italic> mutants show defects in optic fissure closure but not optic fissure formation.

(A-P) Wild type (A-D, I-L) and dzip1ts294e mutants (E-H, M-P) visualized for membranes (Tg(bactin2:EGFP-CAAX)) at 24 hpf (A, E, I, M), 36 hpf (B, F, J, N), 48 hpf (C, G, K, O), and 72 hpf (D, H, L, P). (A-H) Images are lateral views of 3-dimensional renderings. (I-P) Images are lateral views, optical sections from 3-dimensional datasets at the proximal-distal midpoint of the lens. Cyan dashed lines in A-P outline optic fissure margins. At 36 hpf and 48 hpf, dzip1-/- embryos display wider optic fissure openings (F, G, N, O, magenta arrowheads). At 72 hpf, all mutants (9/9) still have an open fissure (P, magenta arrowhead), while 2/15 siblings have an open fissure. (Q-S) Quantification of optic fissure opening angle at 24 hpf, 36 hpf, and 48 hpf. n (embryos) for each genotype shown at the base of the graph. P-values were calculated using Welch’s t-test (Q-S). Scale bar: 50 μm. D, dorsal; V, ventral; N, nasal; T, temporal.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP dzip1ts294e/ts294e; z200Tg standard conditions Prim-5 immature eye plasma membrane IFL
Prim-25 eye plasma membrane IFL
Long-pec eye plasma membrane IFL
Protruding-mouth eye plasma membrane IFL
z200Tg standard conditions Prim-5 immature eye plasma membrane IFL
Prim-25 eye plasma membrane IFL
Long-pec eye plasma membrane IFL
Protruding-mouth eye plasma membrane IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
dzip1ts294e/ts294e; z200Tg standard conditions Prim-5 optic fissure normal width, normal
Prim-5 optic fissure present, normal
Prim-25 eye closure of optic fissure decreased process quality, abnormal
Prim-25 optic fissure closure incomplete, abnormal
Prim-25 optic fissure increased width, abnormal
Long-pec eye closure of optic fissure decreased process quality, abnormal
Long-pec optic fissure closure incomplete, abnormal
Long-pec optic fissure increased width, abnormal
Protruding-mouth eye closure of optic fissure decreased process quality, abnormal
Protruding-mouth optic fissure closure incomplete, abnormal
Protruding-mouth optic fissure open, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One