FIGURE

FIGURE 2

ID

ZDB-FIG-220303-17

Publication

Di Schiavi et al., 2022 - Anosmin-1-Like Effect of UMODL1/Olfactorin on the Chemomigration of Mouse GnRH Neurons and Zebrafish Olfactory Axons Development

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FIGURE 2

Identification of olfactorin in COS-7 cells transfected with the pCMV Sport6.1-FLAG-BQ887653 mouse Umodl1 expression vector. (A) Immunofluorescence detection of olfactorin with an anti-FLAG antibody in COS-7 cells transfected with the pCMV Sport6.1-FLAG-BQ887653 vector. (B) Olfactorin expression in COS-7 cells was evaluated by WB after 48 h of transfection. In protein extracts (olfactorin_FLAG), a full-length olfactorin (148 kDa) detected with olfactorin-serum (olfactorin-Ab) was overexpressed; (C) in the CM of transfected cells the secreted form of olfactorin (62 kDa) was detected by both anti-olfactorin-serum and anti-FLAG-Ab. Experiments were performed independently three times, and a representative blot is shown. C: untrasfected cells; lipo: cells transfected with an empty vector.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol