FIGURE 2

FIGURE 2

Identification of olfactorin in COS-7 cells transfected with the pCMV Sport6.1-FLAG-BQ887653 mouse Umodl1 expression vector. (A) Immunofluorescence detection of olfactorin with an anti-FLAG antibody in COS-7 cells transfected with the pCMV Sport6.1-FLAG-BQ887653 vector. (B) Olfactorin expression in COS-7 cells was evaluated by WB after 48 h of transfection. In protein extracts (olfactorin_FLAG), a full-length olfactorin (148 kDa) detected with olfactorin-serum (olfactorin-Ab) was overexpressed; (C) in the CM of transfected cells the secreted form of olfactorin (62 kDa) was detected by both anti-olfactorin-serum and anti-FLAG-Ab. Experiments were performed independently three times, and a representative blot is shown. C: untrasfected cells; lipo: cells transfected with an empty vector.