FIGURE

Fig. 2

ID

ZDB-FIG-220224-12

Publication

Huang et al., 2021 - Runx1 regulates zebrafish neutrophil maturation via synergistic interaction with c-Myb

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Fig. 2

c-Myb and Runx1 synergistically regulate neutrophil maturation. A–E, SB staining showed further decrease in intensity and number of SB+ cells in 36-hpf c-myb+/−;runx1−/− double mutants (D) compared with single mutants. Red boxes show enlarged detail of SB+ cells in each group (×4). E, quantification of numbers of 36-hpf SB+ cells (each n ≥ 18). (F–I and F’–I’) In vivo VE DIC microscopy revealed further reduction of granules in neutrophils in 2-dpf double mutants (I and I’) compared with single mutants (G, H, G’ and H’) in Tg(mpx:GFP) background (each n ≥ 15). Red arrowheads indicate matured neutrophils with abundant granules. Blue arrowheads indicate immature neutrophils with pale granules. J–K, quantification of absolute numbers of granule abundant-neutrophils per embryo (J) and relative percentage of granule-abundant cells in total mpx:GFP+ neutrophils (K), each n ≥ 15. Scale bars equal 10 μm. L, neutrophil bacterial clearance time in each group. Scale bars, 100 μm (A–D), and 10 μm (F–I and F’–I’).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Biol. Chem.