Fig. 2

Retigabine (RTG), ICA-73, and Wu161 open hKV7.2/7.3 or hKV7.2 channels via different binding sites. A, G(V) curves for hKV7.2/7.3 (expressed in Xenopus oocytes) at pH 7.4 in control solution or RTG as indicated. Data = mean ± SEM. Equation 2: V50 = −44.0 ± 0.6 mV, −76.3 ± 0.4 mV, ΔV50 = −32.3 mV (n = 5). B, G(V) curves for hKV7.2[W236L]/hKV7.3[W265L] (expressed in Xenopus oocytes) at pH 7.4 in control, RTG, or Wu161 as indicated. Data = mean ± SEM. Equation 2 for RTG: V50 = −53.6 ± 2.3 mV, −54.1 ± 2.6 mV; ΔV50 = −0.5 ± 0.3 mV (n = 4); Equation 2 for Wu161: V50 = −51.7 ± 1.5 mV, −69.6 ± 1.5 mV, ΔV50 = −17.8 ± 0.4 mV (n = 5). C, ΔV50 values from A, B, and Figure 1F. Data = mean ± SEM. One-way ANOVA was used with Sidak's multiple comparison test. ns, p > .05; ****, p < .0001. D, G(V) curves for hKV7.2 (expressed in Xenopus oocytes) at pH 7.4 in control solution or ICA-73 as indicated. Data = mean ± SEM. Equation 2: V50 = −43.1 ± 0.3 mV (slope factor s = 8.4 ± 0.3), −105.9 ± 2.5 mV (slope factor s = 20.7 ± 3.0 mV), ΔV50 = −62.8 mV (n = 5). E, G(V) curves for hKV7.2[F168L] (expressed in Xenopus oocytes) at pH 7.4 in control, ICA-73, or Wu161 as indicated. Equation 2 fitted to the data points. ICA-73 (30 µM) : V50 = −28.7 ± 2.2 mV, −30.1 ± 2.3 mV, ΔV50 = −1.4 ± 0.7 (n = 4); 30 µM Wu161: V50 = −32.3 ± 1.9 mV, −60.9 ± 2.7 mV, ΔV50 = −28.6 ± 1.6 (n = 8). F, ΔV50 values from D, F, and Figure 1G. Data = mean ± SEM. One-way ANOVA was used with Sidak's multiple comparison test. ns, p > .05; ****, p < .0001. G, G(V) curves for hKV7.2 (expressed in Xenopus oocytes) at pH 7.4 in control or Wu161 as indicated. Data = mean ± SEM. Equation 2: V50 = −41.1 ± 0.6 mV, −69.4 ± 1.6 mV, ΔV50 = −28.3 ± 1.8 mV (n = 4). Same data as in Figure 1G. H, G(V) curves for hKV7.2 [R198Q] (expressed in Xenopus oocytes) at pH 7.4 in control or Wu161 as indicated. Data = mean ± SEM. Equation 2: V50 −91.1 ± 1.2 mV, −104.1 ± 0.7 mV, ΔV50 = −13.3 ± 1.9 (n = 3). (I) ΔV50 values for data shown in G and H. Data = mean ± SEM. Unpaired t test was used. **, p < .01