FIGURE

Figure 6.

ID

ZDB-FIG-220214-6

Publication

Rovira et al., 2021 - REST is a major negative regulator of endocrine differentiation during pancreas organogenesis

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Figure 6.

REST chemical inhibition in human pancreatic organoids. (A) Western blot analysis of REST FL (full-length) and REST4 protein levels in PANC1 cells treated with X5050 50 µM or DMSO (control). (Lamin B1) Loading control. Bar plot shows the quantification of the Western blot for REST. (B) Human organoids generated from pancreatic exocrine fractions from two cadaveric donors were treated at passage 3 for 48 h with 5 µM X5050 or DMSO (control vehicle). qPCR analysis of mRNA for indicated genes, relative to TBP. Scale bars, 200 µm. Error bars are SD. Student's t-test, (**) P < 0.01.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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