FIGURE

Figure 4.

ID

ZDB-FIG-220131-698

Publication

Mei et al., 2022 - Disruption of PIKFYVE causes congenital cataract in human and zebrafish

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Figure 4.

Detailed characterization of cataract phenotypes in pikfyve^Δ8 mutants.

(A) Confocal imaging of the lens of 5-dpf siblings and pikfyve^Δ8 mutants in Tg(cryaa:DsRed) transgenic background. (B) Hematoxylin-eosin (HE) staining of 5-dpf siblings and pikfyve^Δ8 mutant zebrafish lens after cryostat section. (C) ZL-1 antibody and DAPI staining of 5-dpf siblings and pikfyve^Δ8 mutant zebrafish lens. (D) Transmission electron microscope (TEM) images of the lens of siblings and pikfyve^Δ8 mutants at 3 dpf and 5 dpf. ASS, autophagy lysosome; LD, lipid droplet; N, nucleus. All results were confirmed in three different individuals. All the scale bars represent 10 μm.

Expression Data

Antibody:	zl-1
Fish:	AB pikfyve^shz1/shz1 (AB)
Anatomical Term:	lens fiber cell
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pikfyve^shz1/shz1 (AB)
Observed In:	lens vacuole lens fiber cell
Stage Range:	Protruding-mouth to Day 5

Phenotype Detail

Acknowledgments

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Figure 4.

Figure 4.

Detailed characterization of cataract phenotypes in pikfyveΔ8 mutants.

Detailed characterization of cataract phenotypes in pikfyve^Δ8 mutants.