FIGURE

Figure 6

ID

ZDB-FIG-220131-701

Publication

Mei et al., 2022 - Disruption of PIKFYVE causes congenital cataract in human and zebrafish

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Figure 6

Baf-A1 partially rescued the vacuole defect in the lens of pikfyve^Δ8 mutant zebrafish.

(A) Representative confocal images of the lens of 4-dpf pikfyve^Δ8 mutants treated with DMSO or Baf-A1 for 4.5 hr. (B) Quantification of the vacuole numbers in the lens of 4-dpf siblings and pikfyve^Δ8 mutant embryos treated with DMSO or Baf-A1 (n=10 for sibling groups; n=7 for mutant groups). (C) Confocal images of the lens of 4-dpf pikfyve^Δ8 mutants with no treatment or after 4.5 hr treatment with DMSO or Baf-A1. (D) Quantification of the vacuole numbers in (C) (n=6 for each group). All experiments were repeated three times. All the scale bars represent 20 μm. *, p<0.05; **, p<0.01; ***, p<0.001; ****, p<0.0001, Student’s t-test. See Figure 6—source data 1 for details.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB pikfyve^shz1/shz1 (AB)
Condition:	chemical treatment by environment: Bafilomycin A1(Baf-A1)
Observed In:	lens vacuole
Stage:	Day 4

Phenotype Detail

Acknowledgments

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Figure 6

Figure 6

Baf-A1 partially rescued the vacuole defect in the lens of pikfyveΔ8 mutant zebrafish.

Baf-A1 partially rescued the vacuole defect in the lens of pikfyve^Δ8 mutant zebrafish.