FIGURE 6

Contributions by UG, UGU, and CNNC motifs to Drosha processing and miRNA expression. (A) Comparison of relative Drosha cleavage efficiencies (y-axis) of fruitfly, zebrafish, and human pri-miRNAs with or without the UG, UGU, or CNNC motif (listed on top). Different colors represent different species (x-axis). Columns on the left represent miRNAs without the sequence motif, those on the right with the motif. Integers underneath the columns indicate numbers of miRNAs in the categories. Averages and standard deviations are shown, and the P values shown above the columns, with those <0.05 in red. (B) Normalized expression (y-axis) of fruitfly, zebrafish, and human miRNAs (x-axis) with or without the UG, UGU, or CNNC motif (on top). Labels are the same as in (A). (C) Comparison of relative Drosha cleavage efficiencies (y-axis) of fruitfly, zebrafish, and human pri-miRNAs with 0, 1, 2, or 3 of the UG, UGU, and/or CNNC motifs (x-axis). Labeling is the same as in (A). The various categories are indicated at the bottom, with numbers of the miRNAs listed in parentheses. (D) Normalized expression (y-axis) of fruitfly, zebrafish, and human miRNAs with 0, 1, 2, or 3 of the UG, UGU, and/or CNNC motifs (x-axis). Labels are the same as in (C). (E) Comparison of relative Dicer cleavage efficiencies (y-axis) of fruitfly, zebrafish, and human pre-miRNAs with or without the UGU motif. Different colors represent different species (x-axis). Columns on the left represent miRNAs without the sequence motif, those on the right with the motif. Integers underneath the columns indicate numbers of miRNAs in the categories. Averages and standard deviations are shown, and the P values indicated above the columns.