Fig 4

Jagged1a, Jagged1b and Jagged2a ligands participate in the differentiation of agr2⁺ epidermal mucous cells.(A).Significant increases in agr2⁺ EMC numbers in the trunks and yolks were detected in embryos overexpressing jag1a, jag1b or jag2a mRNA compared to LacZ mRNA-overexpressing embryos at 24 hpf. (B). Significant reductions in agr2⁺ EMC numbers in the trunks and yolks were detected in embryos injected with jag1a UTRMO, jag1a SPMO, jag1b UTRMO or jag2a MO, as well as jag1b and jag2a homozygous mutants compared to wild-type embryos at 24 hpf. (C). Further reductions in agr2⁺ EMC numbers in the trunks and yolks were observed in jag1b homozygous mutant embryos injected with jag1a and jag2a MOs compared to those injected with jag1a or jag2a MO alone or un-injected jag1b homozygous mutant embryos at 24 hpf. Arrows, EMCs. Scale bars, 100 μm. Mean ± SEM. Student’s t-test. *p<0.05; **p<0.01; ***p<0.001; ns, not significant. Underlying data are available in S1 Data.