FIGURE

Figure 2

ID

ZDB-FIG-211216-89

Publication

Farr et al., 2021 - Characterization of the Immune Response to Vibrio cholerae Infection in a Natural Host Model

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Figure 2

Mucin and IL-13 levels increase during V. cholerae infection. WT zebrafish (n=5) were infected with E7946 (El Tor) or 25493 (non-O1) strains of V. cholerae at 2.5 x 10⁷ CFU/mL and then sacrificed at the indicated time points, or water was taken during indicated time points. (A) CFU of the indicated V. cholerae strain taken from zebrafish intestines. (B) Mucin levels in the water of zebrafish infected with the indicated V. cholerae strain. (C) IL_13 expression in fish infected with El Tor strain E7946. (D) IL-13 levels in fish infected with non-O1 strain 25493. Mucin levels were determined via microtiter PAS assay. mRNA levels were determined through qRT-PCR. Gene expression was normalized against β-actin and expressed as fold change. Error bars indicate standard deviation. Data shown is from three experiments. *P < 0.05 as compared to control, **P < 0.05 as compared to 24 h infection.

Expression Data

Gene:	il13
Fish:	AB
Condition:	bacterial treatment by exposure to environment: Vibrio cholerae
Anatomical Term:	intestine
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB
Condition:	bacterial treatment by exposure to environment: Vibrio cholerae
Observed In:	intestine
Stage:	Adult

Phenotype Detail

Acknowledgments

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