ZFIN Image: Sua-Cespedes et al., 2021, FIGURE 1

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FIGURE 1

Experimental design. (A) Danio rerio male adults (n = 144) were divided in two large groups: a control temperature group (animals maintained at 28^oC during 5 days and challenged 6 days at the same temperature) and a lower temperature group (animals maintained at 28^oC during 5 days and challenged 6 days at lower temperature 23^oC). After stimulation the animals were euthanized at early light (EL, 10 AM) and early dark (ED, 12 AM). (B) For the analysis, fish were separated into another two groups: a qPCR (96 animals) and a cortisol (48 animals) group. For the qPCR we evaluated gh1, aanat2, mtnr1aa, mtnr1bb, opn4m1, opn4m2 in the brain, ghra, ghrb, igf1a, igf1b, igf1ra, igf1rb, gr in the liver and muscle. Also, in all tissues analyzed we performed a qPCR for clock genes (per and cry). Each experimental n comprised pooled tissues from four fish. The total number of samples used in each temperature and time point was n = 3–6.

Acknowledgments

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