FIGURE

Fig 1

ID

ZDB-FIG-211116-17

Publication

Mendonca et al., 2021 - Origami: Single-cell 3D shape dynamics oriented along the apico-basal axis of folding epithelia from fluorescence microscopy data

Other Figures

All Figure Page

Back to All Figure Page

Fig 1

Origami Image Analysis Pipeline.

a. Airyscan confocal fluorescence micrograph (maximum intensity projection (MIP) of 35 z-slices) of the developing zebrafish otic vesicle at 51.5 hours post fertilisation. Red box—anterior projection; yellow box—endolymphatic sac; cyan box—posterior projection. The ROIs are expanded alongside—top row MIPs, and bottom row single slices. Scale bars: 20 μm. Blue arrows mark the direction of apicobasal polarity (pointing towards the apical side). b. Polarity assignment on segmented data; ROI surrounding the anterior projection was segmented (here overlaid on the MIP) using ACME, centroids were generated for each segmented cell and a triangular surface mesh was produced from these centroids. Normal vectors (blue arrows) to this surface mesh represent the apico-basal axis. c. Cell shape features were computed concerning the assigned apico-basal axis; here, three example cells are highlighted, alongside a 3D rendering showing their position in the anterior projection and the corresponding shape metrics in a table.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Comput. Biol.