ZFIN Image: Mendonca et al., 2021, Fig 1

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Figure Caption

Fig 1 Origami Image Analysis Pipeline.

a. Airyscan confocal fluorescence micrograph (maximum intensity projection (MIP) of 35 z-slices) of the developing zebrafish otic vesicle at 51.5 hours post fertilisation. Red box—anterior projection; yellow box—endolymphatic sac; cyan box—posterior projection. The ROIs are expanded alongside—top row MIPs, and bottom row single slices. Scale bars: 20 μm. Blue arrows mark the direction of apicobasal polarity (pointing towards the apical side). b. Polarity assignment on segmented data; ROI surrounding the anterior projection was segmented (here overlaid on the MIP) using ACME, centroids were generated for each segmented cell and a triangular surface mesh was produced from these centroids. Normal vectors (blue arrows) to this surface mesh represent the apico-basal axis. c. Cell shape features were computed concerning the assigned apico-basal axis; here, three example cells are highlighted, alongside a 3D rendering showing their position in the anterior projection and the corresponding shape metrics in a table.

Acknowledgments

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