Figure 1

Effect of estradiol (E2) plus lithium chloride (LiCl) on 6-OHDA-induced SH-SY5Y cell death. (a) The SH-SY5Y cells were pretreated with 1 or 5 mM LiCl for 1 hr in the absence or presence of 100 nM E2 and then challenged with 20 μM 6-OHDA for 16 hr. Cell viability of control, 6-OHDA, 6-OHDA plus 1 mM LiCl, 6-OHDA plus 1 mM LiCl and 100 nM E2, 6-OHDA plus 5 mM LiCl, 6-OHDA plus 5 mM LiCl and 100 nM E2, 6-OHDA plus 100 nM E2, 100 nM E2 alone, 1 mM LiCl alone and 5 mM LiCl alone. Cell viability was assessed by AlamarBlue. (b) The SH-SY5Y cells were pretreated with 1 mM LiCl for 1 hr in the absence or presence of 100 nM of E2 and then challenged with 20 μM 6-OHDA for 16 hr. Cell viability of control, 6-OHDA, 6-OHDA plus 1 mM LiCl, 6-OHDA plus 1 mM LiCl and 100 nM E2, and 6-OHDA plus 100 nM E2 by trypan blue. The value corresponds to the mean ± SEM of five independent experiments that have been done in triplicate (n = 15). ^∗p < 0.05 compared with the control group; ^#p < 0.05 compared with the 6-OHDA group.