FIGURE 4

Meiotic recombination pathway showing steps mediated by proteins described in the text. Adapted from Baudat et al., 2013. Red and blue lines represent double strand DNAs of maternal and paternal origins. Broken lines indicate newly synthesized DNA strands during DSB repair. (A) DSB formation requires the topoisomerase-like protein Spo11. (B) The repair of meiotic DSBs involves strand exchange mediated by the ssDNA binding complex RPA, the recombinases Dmc1 and Rad51, and Brca2. (C) Recombination intermediates are resolved to form either crossover or non-crossover products. Crossover resolution involves Mlh1. Other proteins have also been shown to act in meiotic recombination in mammals (reviewed in Baudat et al., 2013) and in budding yeast (reviewed in Yadav and Claeys Bouuaert, 2021). Presented proteins have been studied in zebrafish by mutation and/or cytological analyses. It has not been definitively shown that FANCL/Fancl acts at these stages in both mouse and zebrafish.