FIGURE

Figure 6

ID

ZDB-FIG-210925-40

Publication

Almeida et al., 2021 - Myelination induces axonal hotspots of synaptic vesicle fusion that promote sheath growth

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Figure 6

Chemogenetic stimulation of vesicular fusion promotes sheath growth

(A) Two examples of SypHy activity in TrpV1-tagRFP⁺ reticulospinal axons before (pre-csn) and during capsaicin treatment (+csn).

(B) Capsaicin increased axonal and collateral SypHy in TrpV1⁺ neurons above baseline (difference from zero: p = 0.013 axonal, p = 0.031 collateral, Wilcoxon signed-rank test, 14 axons and 8 collaterals from 14 and 8 animals, respectively).

(D) Single TrpV1⁺ axons treated with vehicle or capsaicin throughout myelination (arrowheads, putative heminodes; n, putative nodes of Ranvier).

(E) Stimulation promotes sheath growth (3–5 dpf change: p = 0.04, Student’s t test). 6 control axons and 5 stimulated axons from 6 and 5 animals, respectively.

Scale bars, 5 μm (A), 10 μm (D), 60 s (A). Graphs in (B) shows median and interquartile range and graphs in (E) display mean and standard deviation.

See also Figure S3 and Videos S7 and S8.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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