FIGURE

Figure 5

ID

ZDB-FIG-210925-39

Publication

Almeida et al., 2021 - Myelination induces axonal hotspots of synaptic vesicle fusion that promote sheath growth

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Figure 5

Axonal vesicular fusion consolidates nascent myelin sheaths

(A and B) Examples of 3–4 dpf time-course analyses of myelination of control RS axons (GFP-2A-tdTcntn1a) and RS axons with blocked vesicular fusion (BoNTB-GFP-2A-tdTcntn1a).

(C) Nascent sheaths in axons with blocked vesicular fusion grow slower (p = 0.048, Mann-Whitney test, 19 control axons from 18 animals and 17 BoNTB axons from 15 animals).

(D) Proportion of axons with and without full sheath retractions (p = 0.038, Fisher’s exact test). Numbers in bars indicate absolute number of axons.

(E) Sheaths >6 μm have a non-significant slower median growth in BoNTB-silenced axons (p = 0.488, Mann-Whitney test, 13 control axons from 13 animals and 14 BoNTB axons from 13 animals).

(F) Proportion of sheaths at 4 dpf fully grown (flanked by nodes and/or collaterals) or still with space to grow (p = 0.001, Fisher’s exact test). Numbers in bars indicate absolute number of sheaths.

Scale bars, 5 μm (A and B). Graphs display median and interquartile range.