Per2 knockout alters the rhythmic mRNA expression of CCGs in adult zebrafish skeletal muscle. qRT-PCR analysis of expression levels of the CCGs (A)hsf2 and (B)myf6 in WT and per2 KO skeletal muscle. Sets of 4 WT and per2 KO fish (each set containing two males and two females) were maintained under LD cycle (14 h light-10 h dark) conditions, muscle tissue was dissected and pooled for RNA extraction at 6-h intervals during a sampling window of 24 h. Mean mRNA relative expression (n = 2–3) ± SD is plotted on the y-axis; zeitgeber time (ZT) is plotted on the x-axis. Zeitgeber times are indicated for each sample. ZT0 corresponds to lights-on, ZT14 to lights-off. A change in rhythm was observed for both CCGs, as determined by a significant ANOVA Genotype × Time interaction effect for all genes (p < 0.001, see Table 1). myf6 exhibited a 6 h phase delay. Asterisks represent levels of significance in comparing the two genotypes at each time point individually, corrected by Sidak’s method (***p < 0.001).
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