Figure 3

Comparative morphological analyses between wild-type fish and tshba mutant fish prior to the juvenile stage. (A) Comparative morphological features and their relationships with age of fish between tshba mutant zebrafish and their wild-type siblings. (B) Comparative morphological features observed and their relationships with fish size between tshba mutant zebrafish and wild-type siblings. The x-axes show developmental milestones describing early larval stages (left) toward late larval stages (right). Measurements were taken of wild-type fish (n = 11) and tshba mutants (n = 10). (C) Morphological features of wild-type and tshba mutants at 16 dpf. Arrows indicate the appearance of inflated anterior swim bladders. (D) Morphological features of wild-type and tshba mutants at 30 dpf. Arrows indicate the appearance of inflated aSBs. (E) Higher magnification views of wild-type fish (30 dpf) showing an inflated aSB (arrows), anterior squamation (SA; details in (e1), red arrowhead), minor fin fold (dotted yellow line, details in (e2) labelled with yellow asterisks), vent, and anal fin. (F) Higher magnification views of tshba mutant fish (30 dpf) showing a smaller inflated aSB (arrows), SA (details in (f1), red arrowhead), minor fin fold (dotted yellow line, details in (f2) labeled with yellow asterisks), vent, and anal fin. Stage abbreviations: CR, caudal fin ray appearance; AC, anal fin condensation; DC, dorsal fin condensation; MMA, metamorphic melanophore appearance; aSB, inflation of anterior swim bladder lobe; AR, anal fin ray appearance; DR, dorsal fin ray appearance; PB, pelvic fin bud appearance; PR, pelvic fin ray appearance; SP, posterior squamation; SA, anterior squamation.** p < 0.01.