Adult zebrafish ECG recording apparatus and protocol. (A) Comparison of human and zebrafish ECG. Top: ECG of a healthy 32-year-old human male, obtained from the MIT-BIH Normal Sinus Rhythm Database (Goldberger et al., 2000). Bottom: ECG from a 6-month-old adult male AB zebrafish, captured using the described recording apparatus and protocol. All cycles within the 60 s (human) and 120 s (zebrafish) recordings were overlaid (green traces), and an average trace was calculated (black trace) using the LabChart ‘ECG Analysis’ module. Traces are shown on the same time scale for direct comparison. (B) Adult zebrafish ECG apparatus; during recordings, the zebrafish is placed ventral side up within the mold located in a well on the base. For ease of reference, apparatus components and axes used to describe the movement of the components are labeled. (C) Summary of adult zebrafish ECG recording protocol. (D) Example from a 6-month-old adult male AB zebrafish using the described recording apparatus and protocol. The trace image was captured using LabChart ‘Zoom View’.
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