FIGURE

FIGURE 7

ID

ZDB-FIG-210828-14

Publication

Islam et al., 2021 - The Zebrafish Meiotic Cohesin Complex Protein Smc1b Is Required for Key Events in Meiotic Prophase I

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FIGURE 7

DNA double strand break marker γ–H2Ax was expressed in smc1b^Q198X mutant testes. Immunolabeling of γ-H2Ax (green) and Telo-FISH (orange) on wild-type and mutant testis sections. (A–D) In wild-type early bouquet stage spermatocytes, γ-H2Ax appeared to localize more broadly in nuclei on testis sections (red dashed lines). Bouquet stage spermatocytes with tightly clustered telomeres (white dashed lines) exhibited γ-H2Ax localization near telomeres. (E–H) Early bouquet-stage spermatocytes in smc1b mutants display broad γ-H2Ax expression similar to wild type, however, tight clustering of telomeres and γ-H2Ax to one side of the nucleus was not observed. Scale bar is 20 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	smc1b^{sa24632/sa24632} (TU)
Observed In:	spermatocyte meiotic DNA double-strand break formation
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol