ZFIN Image: Islam et al., 2021, FIGURE 7

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Figure Caption

FIGURE 7

DNA double strand break marker γ–H2Ax was expressed in smc1b^Q198X mutant testes. Immunolabeling of γ-H2Ax (green) and Telo-FISH (orange) on wild-type and mutant testis sections. (A–D) In wild-type early bouquet stage spermatocytes, γ-H2Ax appeared to localize more broadly in nuclei on testis sections (red dashed lines). Bouquet stage spermatocytes with tightly clustered telomeres (white dashed lines) exhibited γ-H2Ax localization near telomeres. (E–H) Early bouquet-stage spermatocytes in smc1b mutants display broad γ-H2Ax expression similar to wild type, however, tight clustering of telomeres and γ-H2Ax to one side of the nucleus was not observed. Scale bar is 20 μm.

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Acknowledgments

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