FIGURE

FIGURE 9

ID

ZDB-FIG-210828-16

Publication

Islam et al., 2021 - The Zebrafish Meiotic Cohesin Complex Protein Smc1b Is Required for Key Events in Meiotic Prophase I

Other Figures

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FIGURE 9

DNA double strand break and recombination marker Rad51 was expressed in smc1b^Q198X mutant spermatocytes. Immunolabeling of Rad51 (green) and Sycp1 (magenta) in wild-type and mutant spreads, combined with telomere (telo) in situ hybridization (orange). (A,B) Rad51 protein was detected in wild-type beginning in leptotene stage and was initially located near the chromosome ends in leptotene and bouquet stages. (C,D) In more advanced wild-type spermatocytes, Rad51 localization become more distinct and resolved to one or two spots per chromosome. (E,F) Mutant spermatocytes had detectable levels of Rad51. (G) Quantification of Rad51 signal in wild-type (N = 10) and mutant (N = 15) leptotene stage cells demonstrates there is no significant difference (P = 0.0614). The horizontal bars show the medians. Scale bar is 10 μm.

Expression Data

Antibodies:	Ab2-sycp1 Ab5-rad51
Fish:	TU smc1b^{sa24632/sa24632} (TU)
Anatomical Terms:	spermatocyte chromosome spermatocyte nucleus
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	smc1b^{sa24632/sa24632} (TU)
Observed In:	spermatocyte chromosome spermatocyte double-strand break repair involved in meiotic recombination spermatocyte nucleus
Stage:	Adult

Phenotype Detail

Acknowledgments

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