Whole-mount Black-Gold II staining of zebrafish larvae at 7 dpf.(A) Photomicrograph with lateral view and R-C orientation, whole-mount Black-Gold II staining in D + DT + TeO; CeP and SCT at 7 dpf (Unstained CTRL, Solvent CTRL/BG-II and IOP/BG-II), scale bar: 500 μm and 50 μm. In each corner, the amplification of sagittal sections identifying the selected area for analysis and the positive myelin pixels, recognized by the IPP6 program; (B) Graph showing the quantification of myelin density expressed as myelinated area (MA) in pixels2 (individual values and mean ± SEM with n = 5/condition). Statistical analysis was performed with Student’s t test. ***p<0.001, ****p <0.0001.
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