Fig. 5

STIM1 knock-down decreased the expression of HIF-1α, calpain activity and the activity of MMP2 and MMP9 and in ML-1 cells. A The expression of HIF-1α was significantly decreased in STIM1-KD cells compared with MOCK cells. A representative Western blot is shown. B the expression of EMT marker proteins in STIM1-KD cells compared to the MOCK ML-1 cells. The representative Western blots are shown. C the expression of total-ERK and phospho-ERK in STIM1-KD cells compared with MOCK ML-1 cells. The representative Western blots are shown. D STIM1 knock-down decreased the basal and abolished the S1P-evoked increase in calpain activity compared to MOCK ML-1 cells. (N = 4), *Denotes comparison with MOCK. *p < 0.05, **p < 0.01. E The basal and S1P evoked gelatinolytic activity of MMP2 and MMP9 was decreased in STIM1-KD cells, compared with MOCK ML-1 cells. The representative zymography blot is shown. F STIM1 knock-down increased the expression of thyroid-specific proteins. The representative Western blots of at least three independent experiments are shown. G STIM1 knock-down enhanced the basal and TSH-evoked iodine-uptake compared to in the MOCK cells. *Denotes comparison with MOCK cells; o, denotes comparison with control STIM1-KD cells. (n = 4) *p < 0.05, ***p < 0.001, ^oop < 0.01