FIGURE

Figure 4.

ID

ZDB-FIG-210714-54

Publication

Nagar et al., 2021 - The Formin Fmn2b Is Required for the Development of an Excitatory Interneuron Module in the Zebrafish Acoustic Startle Circuit

Other Figures

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Figure 4.

The SAG relaying sensory information and the hindbrain reticulospinal neurons are not affected in Fmn2b morphants. Whole-mount immunostaining with anti-neurofilament antibody 3A10 was used to visualize the SAG connecting to the M-cells in 96-hpf larvae with (A) 2 ng MO control and (B) 2 ng MO SB Fmn2b cytoplasmic injections. Scale bar: 20 μm. Retrograde labeling of reticulospinal neurons using TMR dextran in 96-hpf (C) 2 ng MO control-injected embryos and (D) 2 ng MO SB Fmn2b-injected embryos. The M-cell and its homologs, MiD2cm and MiD3cm, are intact in Fmn2b morphants. Scale bar: 20 μm. The cell bodies of the reticulospinal neurons stained with RMO-44 antibody in 48-hpf morphants are intact in the control and Fmn2b morphants (Extended Data Fig. 4-1).

Expression Data

Antibody:	Ab-3A10
Fish:	WT WT + MO1-fmn2b
Knockdown Reagent:	MO1-fmn2b
Anatomical Term:	statoacoustic (VIII) ganglion neuron projection
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-fmn2b
Knockdown Reagent:	MO1-fmn2b
Observed In:	Mauthner neuron reticulospinal neuron statoacoustic (VIII) ganglion
Stage:	Day 4

Phenotype Detail

Acknowledgments

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