Sensory components of the acoustic startle circuit are not affected in Fmn2b morphants. Hair cells of the lateral crista of the zebrafish inner ear are visualized using the Tg (brn3c:GAP43-GFP) line in 96-hpf larvae with (A) 2 ng MO control and (B) 2 ng MO SB Fmn2b cytoplasmic injections. Scale bar: 10 μm. C, Quantification of the number of hair cell bundles with a kinocilium is depicted in the Gardner–Altman plot. The unpaired median difference between MO control and MO SB Fmn2b is 0.0 [95.0%CI −3.0, 1.0]. The p value of the two-sided permutation t test is 0.687. D, Quantification of kinocilia length in the lateral crista hair cells is shown in the Gardner–Altman plot. The unpaired median difference between MO control and MO SB Fmn2b is 0.247 [95.0%CI −0.851, 1.25]. The p value of the two-sided permutation t test is 0.663. For all the estimation statistics analysis, the effect sizes and CIs are reported as effect size [CI width lower bound; upper bound]. Representative images for FM-4-64 dye uptake assay in the inner ear of 96-hpf (E) control morphants and (F) Fmn2b morphants, done in the background of Tg(cldnb:lyn-GFP) to mark the inner ear boundary. Scale bar: 20 μm.
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