Figure 4.

Figure 4.

The SAG relaying sensory information and the hindbrain reticulospinal neurons are not affected in Fmn2b morphants. Whole-mount immunostaining with anti-neurofilament antibody 3A10 was used to visualize the SAG connecting to the M-cells in 96-hpf larvae with (A) 2 ng MO control and (B) 2 ng MO SB Fmn2b cytoplasmic injections. Scale bar: 20 μm. Retrograde labeling of reticulospinal neurons using TMR dextran in 96-hpf (C) 2 ng MO control-injected embryos and (D) 2 ng MO SB Fmn2b-injected embryos. The M-cell and its homologs, MiD2cm and MiD3cm, are intact in Fmn2b morphants. Scale bar: 20 μm. The cell bodies of the reticulospinal neurons stained with RMO-44 antibody in 48-hpf morphants are intact in the control and Fmn2b morphants (Extended Data Fig. 4-1).