FIGURE

E

ID

ZDB-FIG-210714-45

Publication

Jamalpoor et al., 2021 - Cysteamine-bicalutamide combination therapy corrects proximal tubule phenotype in cystinosis

Other Figures

All Figure Page

Back to All Figure Page

Bicalutamide–cysteamine combination treatment corrects the metabolome and proteome profile of cystinotic proximal tubule cells

Metabolomic analysis of CRISPR‐generated cystinotic cells (CTNS^−/−) treated with cysteamine (100 µM), bicalutamide (35 µM) and a combination of cysteamine and bicalutamide (100 and 35 µM, respectively) (n = 3).

Heat map analysis of the measured metabolites in CTNS^−/− cells upon treatment with cysteamine or cysteamine–bicalutamide combination treatment (n = 3). Metabolites significantly decreased were displayed in green, while metabolites significantly increased were displayed in red.

C, D

Heat map and REACTOME analysis of the altered proteins in CTNS^−/− cells upon treatment with cysteamine, bicalutamide and cysteamine–bicalutamide combination treatment (n = 3). Proteins significantly decreased were displayed in green, while metabolites significantly increased were displayed in red.

Proteomic analysis of CTNS^−/− cells treated with cysteamine, bicalutamide, and a combination of cysteamine and bicalutamide, respectively (n = 3). AKGDH: Alpha‐ketoglutarate dehydrogenase, GLUD1: GLUD2; Mitochondrial glutamate dehydrogenase 1/2, IGF2R; Cation‐independent mannose‐6‐phosphate receptor, GSTK1: Glutathione S‐transferase kappa 1, COX6B1: Cytochrome c oxidase subunit 6B1, ACACA: Acetyl‐CoA carboxylase 1‐Biotin carboxylase, GLS: Glutaminase kidney isoform, mitochondrial, CASP3: Caspase‐3, NPC1: Niemann‐Pick C1 protein.

Data information: Data are expressed as mean ± SEM. P‐values < 0.05 were considered to be significant. One‐way ANOVA with Dunnett’s correction (A,B, C, D and E). * significantly different from CTNS^WT cells (P < 0.05). # significantly different from CTNS^−/− cells (P < 0.05). Exact P‐values and statistical tests are listed in Appendix Table S1.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ EMBO Mol. Med.